查看“MAFFT”的源代码

[https://mafft.cbrc.jp/alignment/software/windows.html 下载地址] 

== 界面 ==
将序列放入MAFFT.bat相同的文件夹中，双击MAFFT.bat<pre>Active code page: 65001
    
Preparing environment to run MAFFT on Windows.
This may take a while, if real-time scanning by anti-virus software is on.                                                                                                                           
It may take a while before the calculation starts                                                                       
if being scanned by anti-virus software.                                                                                
Also consider using a faster version for Windows 10:                                                                    
https://mafft.cbrc.jp/alignment/software/wsl.html                                                                                                                                                                                               
---------------------------------------------------------------------                                                                                                                                                                              
MAFFT v7.450 (2019/Aug/23)                                                                                                                                                                                                                           
MBE 30:772-780 (2013), NAR 30:3059-3066 (2002)                                                                          
https://mafft.cbrc.jp/alignment/software/                                                                       
---------------------------------------------------------------------                                                                                                                                                                                                                                                                                                   
Input file? (FASTA format; Folder=&quot;C:\mafft-7.450-win64-signed\mafft-win&quot;)                                              
@    </pre>

== 参数 ==
@后输入文件名称，如<code>example.fas</code><pre>Output file?
@ </pre>@后输入输出名称，一般喜欢加个out，此处键入<code>exampleout.fas</code><pre>Output format?
  1. Clustal format / Sorted
  2. Clustal format / Input order
  3. Fasta format   / Sorted
  4. Fasta format   / Input order
  5. Phylip format  / Sorted
  6. Phylip format  / Input order
@          </pre>选择<code>4</code><pre>Strategy?
  1. --auto
  2. FFT-NS-1 (fast)
  3. FFT-NS-2 (default)
  4. G-INS-i  (accurate)
  5. L-INS-i  (accurate)
  6. E-INS-i  (accurate)
@         </pre>选择<code>1</code><pre>Additional arguments? (--ep ## --op ## --kappa ## etc)
@      </pre>直接<code>Enter</code><pre>command=
&quot;/usr/bin/mafft&quot;  --auto --inputorder &quot;16S.fas&quot; &gt; &quot;16Sout.fas&quot;
OK?
@ [Y]  </pre>继续<code>Enter</code>，等待结果，到根目录找到example.fas即可
[[分类:Bioinformatics]]