查看“Gblocks”的源代码

[http://molevol.cmima.csic.es/castresana/Gblocks.html 下载地址] 

== 界面 ==
将exampleout.fas移入Gblocks.exe的根文件夹，注意序列文件的名称不能过长或者含有一些非法符号，不然会闪退。<pre>******************************************************
                    GBLOCKS 0.91b
SELECTION OF CONSERVED BLOCKS FROM MULTIPLE ALIGNMENTS
        FOR THEIR USE IN PHYLOGENETIC ANALYSIS
******************************************************

o. Open File

b. Block Parameters

s. Saving Options

g. (Get Blocks)

q. Quit


Your Choice:</pre>

== 参数 ==


选择o<pre>Please enter a DNA or protein alignment in NBRF/PIR or FASTA format or a paths file.
File Name:</pre>输入<code>exampleout.fas</code><pre>******************************************************
                    GBLOCKS 0.91b
SELECTION OF CONSERVED BLOCKS FROM MULTIPLE ALIGNMENTS
        FOR THEIR USE IN PHYLOGENETIC ANALYSIS
******************************************************

CURRENT FILE: exampleout.fas
t. Type Of Sequence: Protein

o. Open File

b. Block Parameters

s. Saving Options

g. Get Blocks

q. Quit


Your Choice:</pre>此时可以选择<code>b</code>更改一些数值

BLOCK PARAMETERS<pre>1. Minimum Number Of Sequences For A Conserved Position: . 21
2. Minimum Number Of Sequences For A Flank Position: ..... 34
3. Maximum Number Of Contiguous Nonconserved Positions: .. 8
4. Minimum Length Of A Block: ............................ 10
5. Allowed Gap Positions: ................................ None

r. Restore Defaults
g. Get Blocks

z. Extended Block Options
m. Go To Main Menu


Your Choice:</pre>输入对应选项可以修改，也可以不修改，此处不修改，直接选择<code>g</code><pre>exampleout.fas
Original alignment: 1085 positions
Gblocks alignment:  81 positions (7 %) in 4 selected block(s)</pre>此时根目录里<code>exampleout.fas-gb.htm</code>是保守区选择信息，将<code>exampleout.fas-gb</code>修改为<code>exampleoutgb.fas</code>或者其它以<code>fas</code>结尾的文件，得到了选择了保守区的序列。
[[分类:Bioinformatics]]